Spectrophotometric Determination of Glutamine Synthetase Activity in Cultured Cells
نویسندگان
چکیده
منابع مشابه
Regulation of glutamine synthetase in cultured Chinese hamster cells. Induction and repression by glutamine.
Glutamine synthetase activity in Chinese hamster cells in tissue culture possesses properties identical with purified liver glutamine synthetase. The cellular level of enzyme is regulated by the concentration of glutamine in the growth media. Induction by removal of glutamine leads to an 8to IO-fold increase in enzyme activity within 48 hours. Repression is a much more rapid process. Half of th...
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Most hepatomas contain only traces of glutamine synthetase (GS) although they originate from liver which has high levels of the enzyme (1-3) The H T C cell line which was derived from Morris Hepa toma 7288C retained certain characteristics of liver cells, notably the induction of tyrosine amino transferase (4, 5), while other liver characteristics, among them GS activity, could not be detected ...
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The use of phosphoenolpyruvate plus pyruvate kinase as an ATP-generating system in the assay for glutamine synthetase activity via the formation of gamma-glutamylhydroxamate from glutamate and hydroxylamine with crude tissue preparations is shown to give values far in excess of the true glutamine synthetase activity of the tissue. This is due to the generation of pyruvate, which reacts with hyd...
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Changes in thymidylate synthetase specific activity in Don Chinese hamster cells grown in vitro have been examined during the culture cycle and after exposure of lagand log-phase cultures to drugs which inhibit DNA, RNA, and protein synthesis. During the culture cycle enzyme activity was low during lag phase, rose 6to 8-fold before log phase, fluctuated between 5-5 and 9 nmol dTMP/h/10 cells du...
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Regulation of glutamine synthetase activity was studied in a glutamine-independent subclone of the hepatoma tissue culture cell line. In cells of this subclone glutamine synthetase activity was elevated by the synthetic corticosteroid hormone, dexamethasone, and depressed by glutamine. The increase in glutamine synthetase activity due to the addition of dexamethasone and that due to removal of ...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2016
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.1959